Journal: Burns & Trauma

Article Title: Tripartite motif 13 orchestrates endoplasmic reticulum-associated degradation and endoplasmic reticulum-phagy to modulate dendritic cell-mediated immune responses in sepsis

doi: 10.1093/burnst/tkaf077

Figure Lengend Snippet: TRIM13 promotes SEL1L-HRD1 endoplasmic reticulum-associated degradation (ERAD) and ER-selective autophagy (ER-phagy) in DCs upon LPS stimulation. (a) Immunoblot analysis of ER-phagy-related protein expression in WT and Trim13 Cd11c DCs at the indicated post-CLP time points ( n = 3). The quantitative results are shown on the right. (b) Immunoblot analysis of ERAD-related protein expression in WT and Trim13 Cd11c DCs at the indicated post-CLP time points (n = 3) ( n = 3). The quantitative results are shown on the right. (c) Immunoblot analysis of ER-phagy-related protein expression in Trim13 KD and Trim13 OE DC2.4 cells following stimulation with LPS (1 µg/ml, 24 h) (n =3). The quantitative results are shown on the right. (d) Immunoblot analysis of ERAD-related protein expression in Trim13 KD and Trim13 OE DC2.4 cells following stimulation with LPS (1 μg/ml, 24 h) ( n = 3). The quantitative results are shown below. (e) Immunoblot analysis of STING ubiquitination following STING immunoprecipitation in Trim13 OE DC2.4 cells treated with MG-132 (10 μm, 12 h) or Hrd1 knockdown. Data are expressed as means ± SD. ns = not significant; * P < 0.05; ** P < 0.01; *** P < 0.001. DCs dendritic cells, SD standard deviation, ER endoplasmic reticulum, WT wild-type, TRIM13 tripartite motif 13, CLP cecal ligation and puncture, STING stimulator of interferon genes

Article Snippet: HRD1/SYVN1 (13473–1-AP) and SEL1L (29801-1-AP) antibodies were purchased from Proteintech (Wuhan, China).

Techniques: Western Blot, Expressing, Ubiquitin Proteomics, Immunoprecipitation, Knockdown, Standard Deviation, Ligation